E64D is a cell permeable derivative of E64-C, an inhibitor of calpain and other cysteine proteases, such as papain, cathepsin B and cathepsin L. Addition of E64D to cell cultures should be done in serum-free media, as esterases in serum will cleave the ethyl ester and reduce cell permeability. Typical working concentration of E64D is 0.5-10 μg/ml. E64D inhibits degradation of autophagic cargo inside autolysosomes.
|Purity||98.0% by HPLC|
|Quantity||1 mg, 5 mg|
|Molecular Weight||342.4 g/mol|
|Physical State||Lyophilized white to off-white powder|
|Solubility||20 mg/mL in DMSO, 10mg/mL in ethanol|
|Storage||-20°C, avoid freeze/thaw cycles|