Other protein expression and purification systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest. LifeSensors’ highly active and specific SUMOstar Protease 1 recognizes the tertiary structure of the SUMOstar tag, generating the desired N-terminus and never cleaving within your protein of interest. In addition the SUMOstar system makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMOstar fusion tag and SUMOstar Protease 1, the SUMOstar system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.
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|Promoter||T7 RNA polymerase|
|Host||E. coli, yeast|
|Storage||Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.|