The SUMOpro Protein and Peptide Expression Kit maximizes yields of soluble, untagged, functional proteins with the desired N-terminus. While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO protease 1 recognizes the tertiary structure of the SUMO tag, generating the desired N-terminus and never cleaving within your protein of interest. In addition, the SUMOpro kit makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMO fusion tag and SUMO Protease 1, the SUMOpro system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.
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-pE-SUMO Kan vector (Cat.# 1001K) – 20 µg
-SUMO Protease 1 (Cat.# 4010) – 500 Units
-SUMO Protease 1 control protein (Cat.# 5000) – 100 µg
-Anti-SUMO/SUMOstar antibody (Cat.# AB7002) – 50 µg
|Promoter||T7 RNA polymerase|
|Selection marker||Kanamycin (E. coli)|