1000K: pE-SUMOpro Kan Kit

$1,365.00

In stock

Specifications

The SUMOpro Protein and Peptide Expression Kit maximizes yields of soluble, untagged, functional proteins with the desired N-terminus. While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO protease 1 recognizes the tertiary structure of the SUMO tag, generating the desired N-terminus and never cleaving within your protein of interest. In addition, the SUMOpro kit makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMO fusion tag and SUMO Protease 1, the SUMOpro system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.

This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Kit Components:
-pE-SUMO Kan vector (Cat.# 1001K) – 20 µg
-SUMO Protease 1 (Cat.# 4010) – 500 Units
-SUMO Protease 1 control protein (Cat.# 5000) – 100 µg
-Anti-SUMO/SUMOstar antibody (Cat.# AB7002) – 50 µg

Fusion tag  His6-SUMO 
Quantity  n/a
Promoter  T7 RNA polymerase 
Selection marker  Kanamycin (E. coli) 
Host  E. coli 
Storage 
Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

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Applications

  • High-yield production of untagged recombinant proteins in E. coli


Benefits

  • SUMOpro may dramatically enhance the solubility of recombinant proteins in E. coli
  • Specific cleavage of SUMO yields native protein with desired N-terminus (except proline)
  • SUMOpro has been shown to protect peptides from intracellular degradation

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