1000A: pE-SUMOpro Amp Kit


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SKU: PE-1000-A100 Category:


The SUMOpro Protein and Peptide Expression Kit maximizes yields of soluble, untagged, functional proteins with the desired N-terminus. While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO protease 1 recognizes the tertiary structure of the SUMO tag. This generates the desired N-terminus and never cleaves within your protein of interest. The SUMOpro kit makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMO fusion tag and SUMO Protease 1, the SUMOpro system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.

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Kit Components:
-pE-SUMO Amp vector (Cat.# 1001A)- 20 µg
-SUMO Protease 1 (Cat.# 4010)-500 Units
-SUMO Protease control protein (Cat.# 5000) – 100 µg
-Anti-SUMO/SUMOstar antibody (Cat.# AB7002) – 50 µg

Fusion tag  His6-SUMO
Quantity   n/a
Promoter  T7 RNA polymerase 
Selection marker  Ampicillin (E. coli) 
Host  E. coli 
Storage  Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

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  • SUMOpro may dramatically enhance the solubility of recombinant proteins in E. coli
  • Specific cleavage of SUMO yields native protein with desired N-terminus (except proline)
  • SUMOpro has been shown to protect peptides from intracellular degradation