Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitylated proteins, allowing detection at relatively low abundance. TUBEs effectively “capture” proteins in their polyubiquitylated state.
His6 Anti-K63 TUBE can be used to isolate, enrich, and identify ubiquitylated proteins from cells, tissues, and organs.
|Molecular Weight||37,825 Da|
- “Far Western” blot analysis
- Histo- or cyto-chemical staining. N.b. Sample fixation will require optimization.