1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn2+ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.
|Molecular Weight||198.21 Da|
|State||0.5 M DMSO solution, 100X|
|Storage||-80°C Product can be shipped at room temperature.|
- A critical component for protecting K63-linked polyubiquitin chains in cell lysates.