pI-secSUMOstar is used for expression and secretion of SUMOstar fusion proteins (containing an N-terminal His6 tag) in baculovirus expression vector systems (BEVS). Bacmid generation from this plasmid requires the use of DH10bac for transposition into the baculoviral genome. Expression of fusion proteins is driven by the late stage viral polyhedrin promoter (polh). Your protein of interest (POI) is targeted for processing and secretion through fusion with the secretion signal sequence from the envelope surface glycoprotein 67 (gp67) of Autographa californica. pI-secSUMOstar contains an ampicillin marker for propagation in E. coli.
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|Selection marker (bacterial)||Ampicillin|
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