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LifeSensors

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4110-250UNITS SUMOstar Protease

$250.00
  • Product Code: 4110-250UNITS SUMOstar Protease
  • Availability: In Stock

Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems (Cat.#s: 1100A11061100K11012100210121602161310031017110711171207121) . Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2.

By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

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Info


Molecular weight 26 kDa
Fusion tag His6
Source E. coli
Purity >95%
Physical state Liquid
Activity >105 Unit/mg
1U of SUMOstar Protease 1 cleaves >90% of 100 ug SUMOstar Protease 1 Control Protein in 1 hour (30°C)
Storage/Stability Store at -80°C, avoiding repeated freeze-thaw cycles. Stable for up to one year under these conditions.



Applications


  • SUMOstar Protease 1 contains a polyhistidine tag at the N-terminus, allowing easy removal from the cleavage reaction by affinity chromatography. The protease cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5-9.5], and ionic strength.
  • Specifically engineered to recognize and cleave the SUMOstar fusion tag


Documents

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