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LifeSensors

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4020-1,000UNITS SUMO Protease 2

$795.00
  • Product Code: 4020-1,000UNITS SUMO Protease 2
  • Availability: In Stock

For removal of SUMOpro3 tags from fusion proteins expressed from the following SUMOpro3 expression systems: (Cat.#s: 1010A, 1016, 1010K, 1011, 2150, 2151). SUMO Protease 2, a highly active and robust recombinant protease, cleaves hSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3. As a result, SUMO Protease 2 will not cleave within the fused protein of interest.

By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

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Info


pfam Peptidase C-48
Molecular weight 28 kDa
Fusion tag His6
Source E. coli
Purity >95%
Physical state 10 U/µL in 50 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol
Activity >105 Unit/mgcore
1U of SUMO Protease 1 cleaves >90% of 100 µg SUMO Protease 1 Control Protein in 1hour (30°C)
Storage/Stability Store at -80°C, avoiding repeated freeze-thaw cycles. Stable for up to one year under these conditions.



Applications


  • Digestion and removal of SUMOpro tags from recombinant fusion proteins made with our SUMOpro Expression systems (Cat.#s: 1010A10161010K101121502151)


Benefits

  • SUMO Protease 1 cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5-9.5], and ionic strength. SUMO Protease 1 contains a polyhistidine tag at the N-terminus, making it easy to remove from the cleavage reaction by affinity chromatography.


Documents

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