The FK2 monoclonal ubiquitin antibody is a frequently used and widely-cited ubiquitin antibody that detects both mono- and polyubiquitin, but not free ubiquitin. Raised against a crude preparation of polyubiquitinylated lysozyme, it has been shown to recognize K11-, K29-, K48- and K63-linked polyubiquitin and to perform in Western blotting, immunoprecipitation, and immunostaining applications.
For comparative studies, LifeSensors has developed several monoclonal antibodies that recognize polyubiquitylated proteins and free ubiquitin. Like FK2, the VU-1 Ubiquitin Monoclonal Antibody has been shown to recognize different linkages including K48-, K63-, and K11-polyubiquitylated conjugates. In contrast, VU-1 recognizes free ubiquitin and linear ubiquitin chains and is an excellent reagent to use for immunofluorescence in tissue sections and cell culture. If you are not performing immunopercipitaion experiments, the VU-1 antibody is a better choice.
|Purity||>90% by SDS-PAGE|
|Production||Purified from ascites using ion exchange chromatography|
|Concentration||10 mg/mL in 10mM phosphate buffer, 0.15 M NaCl, pH 7.4, 0.1% Sodium Azide|
|Storage||Store at -20°C, avoid repeated freeze/thaw cycles|
- Due to the high conservation of ubiquitin across eukaryotes, the specificity is independent of species