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LifeSensors

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UE103 UbiQuant (TM) S

$695.00
  • Product Code: UE103 UbiQuant (TM) S
  • Availability: In Stock

To date, detecting changes in the ubiquitylation of specific substrate proteins in response to external stimuli, e.g. stress, cytokine exposure, drug candidate treatment, etc., has been a long, labor- intensive process involving immunoprecipitation followed by gel electrophoresis and Western blot analysis.  This method is low through-put, resource intensive, and only semi-quantitative at best.  We have developed the UbiQuant™ S kit as a facile, robust, and quantitative alternative to IP/WB analysis. Built on LifeSensors UbiQuant platform, the system captures ubiquitylated proteins in the wells of a microtiter plate and then uses an antibody to the protein of interest (either native or tagged) to quantitate the amount of the protein bound.


Note: If using a tagged-substrate, the user must specify the epitope tag in order to receive the appropriate antibody for detection (e.g. myc, HA, FLAG®, V5, GST, etc.  Unfortunately, His6 cannot be used for this assay.)

Applications
  • Determine changes in the level of substrate ubiquitylation as a result of cell treatment.
  • Uses an antibody to the protein of interest. 


Benefits
  • Medium throughput, quantitative method.


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