LifeSensors has developed TUBE (Tandem Ubiquitin Binding Entity) technology for the detection, characterization and isolation of polyubiquitylated proteins from cells and tissue extracts. TUBEs capitalize on the linkage of multiple ubiquitin interacting motifs (UIMs) to generate reagents with high affinity for polyubiquitin.TUBEs have 100 to 1000-fold higher affinity for polyubiquitin chains compared to monomer ubiquitin binding domains (UBDs). TUBEs both stabilize and bind to ubiquitylated proteins, serving as an indispensable tool for ubiquitologists. Our versatile collection of TUBEs includes reagents for the isolation/pulldown of polyubiquitin conjugates include both linkage-specific and non-specific reagents including Anti-M1 (Linear) TUBE, Anti-K63 TUBE, Anti-K48 TUBE, and the non-specific reagents Anti-Ubiquitin TUBE1 and TUBE2.
We have di-, tri-, and tetra- M1 (linear) ubiquitin substrates that are resistant to cleavage by Otulin, which can be used as controls or in competition based experiments. Additionally, we sell native linear chains. Our LUB9 antibody can be used to detect linear ubiquitylated substrates after pull down with TUBES.
This product is protected by one or more US or Foreign patents.
|Buffer||PBS, pH 7.2; 5% glycerol|
|Molecular weight||33.4 kDa|
|Storage||Stable for up to 1 year at -80ºC. Aliquot reagent and avoid repeated freeze/thaw cycles.|
- Isolation and enrichment of M1-polyubiquitinated proteins from cell and tissue extracts
- Isolation of ubiquitylated proteins for proteomic studies
- Nanomolar dissociation constant (Kd) for M1-chains
- 1000 to 10,000-fold preference for M1 chains over K48- or K63- chains
- Overexpression of epitope-tagged ubiquitin for pull downs is not necessary
- Compatible with Ni+2IMAC, streptavidin, or FLAG® -based isolation technologies, providing flexibility and specificity
|UM206_HisM1TUB E.pdf File Size 71.98KB Downloaded: 0|
|Anti-M1 TUBE M anual_122215.pdf File Size 425.01KB Downloaded: 0|