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UM407M - 1ML K48 TUBE HF, Magnetic

  • Product Code: UM407M - 1ML K48 TUBE HF, Magnetic
  • Availability: In Stock

TUBEs:  A Revolution in Polyubiquitin Isolation and Characterization
The use of Tandem Ubiquitin Binding Entities (TUBEs) is emerging as an indispensable strategy for ubiquitin research. The first generation of these TUBEs bind K48- and K63-linked tetraUb chains with single digit nanomolar Kds, ~100 to 1000-fold more tightly than monomeric UBAs. TUBEs also protect proteins from DUBs and the proteasome, even in the absence of inhibitors normally required to block such activity. This allows efficient isolation of native polyUb chains and attached proteins from cell lines, tissues, and organs under conditions that are less likely to alter cell physiology than those listed above. TUBE1 and TUBE2 have recently been demonstrated to enrich for all polyUb chain linkage types, without discrimination, making these reagents appropriate even if the linkage type is not known for the protein of interest.
The next generation of TUBEs:  Linkage Specific Isolation of PolyUb Chains
K48 TUBE HF was developed to show enhanced selectivity for K48-linked polyubiquitin chains (~20 nM) over all other linkages (>2 µM). It can be used alone or in conjunction with our other TUBE products, especially K63 TUBE and M1 (linear) TUBE to investigate polyubiquitin chain linkage in your substrate protein.
Note: The new K48 TUBE HF has a comparable affinity as our existing K63 TUBE. Both of them bind to their cognate Ub4s with a Kd of ~ 10-20 nM.
Magnetic-TUBEs are TUBE moieties directly coupled to magnetic beads, for the identification and characterization of polyubiquitinated proteins by western blotting and/or downstream proteomic studies. Magnetic-TUBEs facilitate convenient "one-step" pull-down of polyubiquitinated proteins.

K48 TUBE HF, Magnetic
Size: 1ml
Buffer: PBS, pH 7.2, 20% ethanol
Storage: Stable for up to 6 months at 4oC. Do not centrifuge at high speeds (>3000g), dry or freeze the beads.
Isolation and enrichment of K48-polyubiquitinated proteins from cell and tissue extracts
Isolate K48-polyubiquitinated proteins for proteomic studies

100-fold preference for K48 poly-ubiquitin chains over all other linkages
TUBEs display higher affinity towards polyubiquitins than most ubiquitin antibodies
Avoids overexpression of epitope-tagged ubiquitin for pull downs

Thumb File information
UM407M_K48TUBE HF Magnetic M SDS 2018.pdf 224.09KB 0
UM407M_K48TUBE HF Magnetic M anual 2018.pdf 211.5KB 0

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