SUMO system FAQs
What is the advantage of using the SUMO system?
Many engineered/designer proteins or
single-chain antibodies are poorly expressed. Fusion with SUMO/SUMOstar
enhances the stability, solubility, and yield of a protein. SUMO is one of the
most conserved proteins in eukaryotes and have been shown to post-translationally
modify a number of proteins in cells. SUMOylation plays an important role in
various cellular process such as nuclear-cytosolic transport, transcriptional
regulation, and protein stability. SUMO/SUMOstar-fusion leads to a dramatic
enhancement of expression in E. coli, yeast, insect, and mammalian cells and
promotes correct folding and hence biological activity. Additionally, this
system simplifies and lowers the costs associated with protein expression and
purification. Using this technology, a plethora of proteins have been shown to
enhance their expression, stability, and yield.
What is the difference between SUMOpro and
SUMOstar extends the benefits of our SUMO
system to eukaryotes. Therefore SUMOstar is
preferred expression system for all difficult to express proteins in
mammalian and other eukaryotic cells. SUMOstar fusion cannot be cleaved with
SUMOprotease or SUMO protease II as the tag is engineered not to be cleaved.
The SUMOstar tag can only be cleaved with SUMOstar protease.
What host models are available?
We offer systems that are optimized for:
Yeast: S.cereviseaand P.pichia
Baculoviral/Insect cell system
Mammalian: HEK293and CHO cells
What is the SUMOpro3 system?
LifeSensors discovered that
human SUMO is superior for E.coli expression of some of the proteins in E.coli.
Therefore we developed human SUMO system and called it SUMOpro3. SUMOpro3 tag
is best cleaved with SUMO protease 2, that is a human de-SUMOylase. Please contact us to help you evaluate the best system for your specific
What is included in a kit?
Our SUMO system kit offers everything you
need to express your protein of interest in a given host. The kits include a
SUMO plasmid where you can insert your gene of interest, the SUMO protease to
cleave the SUMO tag, the SUMO protease control protein, and an antibody to
detect our SUMO tag. The kits include a manual featuring a polylinker map of
the vector for an easy cloning process.
What is the best way to ensure the protease
removed the SUMO tag?
LifeSensors offers an antibody to detect the SUMO tag. This allows you to check the efficiency of the protease cleavage by simple western blot methods. We recommend using this as a quality checkpoint when optimizing your expression system and protein purification. We are happy to help you implement best practices for protein expression and provide recommendation to get a high yield of active