TUBE-based Mass Spectrometry Proteomics
TUBE-based enrichment of polyubiquitinated proteins has proven key for the progress of ubiquitin proteomics. LifeSensors have developed two proprietary technologies to- (1) identify cellular proteins that are ubiquitinated (TUBE proteomics), and (2) qualitative and quantitative profiling of ubiquitination sites on proteins (UbiSight proteomics). A brief overview of these two technologies are depicted in the following figure.
LifeSensors’ pan (TUBE1 and TUBE2) as well as linkage selective (M1-, K48-, and K63) TUBEs provide isolation of respective polyubiquitinated proteins. The TUBE enrichment kit contains solutions to provide simple procedure to lyse cells, wash the non-specific binding proteins, and elute proteins in solution compatible for further protease digestion. TUBE proteomics services include following four steps:
- TUBE Enrichment Kit - Kit for enrichment of polyubiquitinated protein: Magnetic-TUBE1 and TUBE2 beads, Cell lysis buffer, Wash buffer and Elution buffer
- K48 TUBE Enrichment Kit - Kit for enrichment of K48-polyubiquitinated proteins: K48-linkage selective TUBE, Cell lysis buffer, Wash buffer and Elution buffer
- K63 TUBE Enrichment Kit - Kit for enrichment of K63-polyubiquitinated proteins: K63-linkage selective TUBE, Cell lysis buffer, Wash buffer and Elution buffer
- M1 TUBE Enrichment Kit- Kit for enrichment of M1 (linear)-polyubiquitinated proteins: M1-linkage selective TUBE, Cell lysis buffer, Wash buffer and Elution buffer