Deubiquitylases (DUBs) are enzymes that can reverse the effect of E3 ligases by removing the ubiquitin from the target proteins. Unlike most E3 ligases, DUBs have inherent catalytic activity that is easily targeted by small molecules. These same DUBs are involved in ubiquitination maturation, recycling, and editing. There are over 100 DUBs that can be divided into seven subfamilies, all capable of being encoded by the human genome. These DUBs have been implicated in many regulatory mechanisms, and biological functions, where they play a role in a plethora of clinical diseases. Here at Lifesensors, we offer you products, technology, and services that help you study DUBs.

Lifesensors has developed a variety of novel fluorescent-based DUB assays, utilizing physiological substrates and polyubiquitin isopeptide-selective substrates. The C-terminus of ubiquitin is tagged with a quenched fluorogenic substrate upon cleavage by the DUB, thus reporting DUB activity. We are proudly the only company that has a wide variety of tools with patented assay platforms that can characterize DUB inhibitor compounds and demonstrate selectivity using carefully curated DUB selectivity panels. We have expressed and purified 35+ DUBs that are validated in HTS and compound profiling. We have established DUB-centric compound libraries to provide standards for monitoring the inhibition of your compounds. Additionally, you will find we offer products and services with di-, tri-, and tetra- ubiquitin chains of different linkages tailored for your studies, along with DUB resistant poly ubiquitin chains to investigate binding interactions.     

Associated Services

Ubiquitin Fluorophore Assay

Utilizes a ubiquitin molecule bound to a quenched fluorophore. Upon cleavage, the fluorophore fluoresces, and the signal is measured.

CHOP assay

Ubiquitin is fused to a reporter enzyme on the N-terminus of the reporter and the C-terminus of the ubiquitin, rendering the reporter inactive. Upon cleavage via the DUB the reporter is free and can fluoresce.

IQF Assay

This assay involves a pair of conjugated ubiquitin proteins. One carries a fluorophore and is quenched by the other. When the DUB cleaves the di-ubiquitin the fluorophore is no longer quenched and can be detected.