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UB204H-3 NMOLES UBE2K (UbcH1), His6

$165.00
  • Product Code: UB204H-3 NMOLES UBE2K (UbcH1), His6
  • Availability: In Stock

UbcH1 (also known as E2-25K) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Once an E1 is carrying an activated ubiquitin in a ubiquitin-E1 thiolester complex, this activated ubiquitin is transferred to the active site cysteine of the E2 to form a ubiquitin-E2 thiolester complex. With the aid of an E3, the activated ubiquitin is then targeted to a specific substrate lysine. UbcH1 is a biochemically important E2 due to its ability to form free K48-linked polyubiquitin chains in solution in the absence of an E3. This ability is attributed to UbcH1 possessing a C-terminal UBA domain, in addition to its E2 catalytic domain.

Info
Amount 3 nmol
State Liquid
Molecular Weight 23,400.7 Da
Source Human Recombinant
Fusion Tag His6
Accession Number P61086
Alternate Names E2-25K, UbcH1, HIP2, Ubc1
Storage -80°C, avoid freeze/thaw cycles
Stability 1 year under recommended storage conditions
Recommended Use Enzyme concentration of 100 nM – 1 mM is recommended for in vitro conjugation.



Applications
  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes


Benefits
  • Untagged (native) E2 conjugation enzyme


Documents

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