You are using an outdated browser. For a faster, safer browsing experience, upgrade for free today.

LifeSensors

Call us: 610.644.8845

UB204H-0.8 NMOLES UBE2K (UbcH1), His6

$41.25
  • Product Code: UB204H-.8 NMOLES UBE2K (UbcH1), His6
  • Availability: In Stock

UbcH1 (also known as E2-25K) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Once an E1 is carrying an activated ubiquitin in a ubiquitin-E1 thiolester complex, this activated ubiquitin is transferred to the active site cysteine of the E2 to form a ubiquitin-E2 thiolester complex. With the aid of an E3, the activated ubiquitin is then targeted to a specific substrate lysine. UbcH1 is a biochemically important E2 due to its ability to form free K48-linked polyubiquitin chains in solution in the absence of an E3. This ability is attributed to UbcH1 possessing a C-terminal UBA domain, in addition to its E2 catalytic domain.

Info
Amount 0.8 nmol
State Liquid
Molecular Weight 23,400.7 Da
Source Human Recombinant
Fusion Tag His6
Accession Number P61086
Alternate Names E2-25K, UbcH1, HIP2, Ubc1
Storage -80°C, avoid freeze/thaw cycles
Stability 1 year under recommended storage conditions
Recommended Use Enzyme concentration of 100 nM – 1 mM is recommended for in vitro conjugation.



Applications
  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes


Benefits
  • Untagged (native) E2 conjugation enzyme


Documents

Write a review

Note: HTML is not translated!
    Bad           Good