E6-AP (E6-associated protein) is an E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. It is a HECT domain E3 containing an active site cysteine which accepts a charged ubiquitin from the ubiquitin-E2 thiolester complex. E6-AP has been linked to a neurodevelopmental disorder, Angelman syndrome. In addition the oncoprotein E6 of human papillomaviruses (HPV) utilizes E6-AP to target numerous cellular proteins for degradation including the tumor suppressor protein p53.
|Molecular Weight||101 kDa|
|Storage||Store at -80°C, avoiding repeated freeze-thaw cycles.|
|Alternative Names||Ubiquitin-protein ligase E3A, UBE3A|
- Typical enzyme concentration of 100 nM – 5 µM is used for in vitro conjugation, depending on conditions.