DeNeddylases

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Den1

Catalog Number
DB801
Molecular Weight
51kDa
Family
Deneddylase
Physical State
Liquid
Purity
>90%
Alternate Name(s)
DEN1, Deneddylase-1, FKSG8, HsT17512, NEDD8-specific protease 1, NEDP1, Protease, cysteine 2, PRSC2, Sentrin/SUMO-specific protease SENP8, Sentrin-specific protease 8
Fusion Tag
GST
Source
Human Recombinant
Package Size
25ug
Active
Yes

Product Information

Price

$195.00 USD

Storage/Stability
References

Documentation

Datasheet

Product Description

DEN1 (SENP8, NEDP1) is a deNEDDylase that deconjugates NEDD8 conjugates1-3. A recent study demonstrated that DEN1 deNEDDylated the tumor suppressor breast cancer-associated protein BCA34. Full length DEN1 cleaves NEDD8-PLA2 with high efficiency.

 

Activity: This enzyme is active in the NEDD8-IsoPro assay.

 

Storage/Stability

Store at -80°C, avoiding repeated freeze-thaw cycles.

 

References

1: Mendoza, H.M., et al., NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins. J Biol Chem, 2003. 278(28): p. 25637-43.
2: Gan-Erdene, T., et al., Identification and characterization of DEN1, a deneddylase of the ULP family. J Biol Chem, 2003. 278(31): p. 28892-900.
3: Wu, K., et al., DEN1 Is a Dual Function Protease Capable of Processing the C Terminus of Nedd8 and Deconjugating Hyper-neddylated CUL1. J. Biol. Chem., 2003. 278(31): p. 28882-28891.
4: Gao, F., et al., Neddylation of a breast cancer-associated protein recruits a class III histone deacetylase that represses NFkappaB-dependent transcription. Nat Cell Biol, 2006. 8(10): p. 1171-7.