Insect Secretory SUMOstar Expression Kit

Product Information

Benefits
Storage/Stability
Components

Documentation

Manual
Polylinker Map
White Paper

Product Description

In eukaryotes, endogenous SUMO proteases rapidly cleave SUMO fusions in vivo, precluding tag-enhanced expression and purification capacities. To circumvent this problem, the SUMOstar fusion tag and novel protease were engineered. SUMOstar offers the same solubility and expression enhancing benefits as the original wild-type SUMO tag; however, the tag is not cleaved by endogenous eukaryotic SUMO proteases. As a result, endogenous SUMO proteases do not cleave the SUMOstar fusion construct. A specific SUMOstar protease was subsequently engineered to specifically recognize and cleave the SUMOstar tag in vitro, liberating the protein-of-interest with a desired N-terminal amino acid.

Benefits

• Fusion proteins are targeted to the secretory pathway for folding and secretion.

• SUMOstar fusion enhances the expression level and solubility of fusion proteins in eukaryotic expression system.

• The SUMOstar tag protects the expression host from the cytotoxic effects of many protein fusion partners.

• SUMOstar Protease 1 is specifically engineered to recognize and cleave the SUMOstar fusion tag to release the protein-of-interest with a desired N-terminus.

• The insect expression system offers an alternative to yeast expression systems, and often produces more functional protein through high-fidelity post-translational modifications and chaperone-mediated protein folding.

• Insect expression provides a cost effective eukaryotic alternative to mammalian expression systems for functional protein production.

   

Storage/Stability

Store all components at -20°C, avoiding repeated freeze-thaw cycles. For long-term use, store SUMOstar Protease 1 at -80°C.

Components

1. pI-secSUMOstar Vector

2. SUMOstar Antibody

3. SUMOstar Protease 1

4. SUMOstar Control Protein